Publications Edmonds, A. K., Balourdas, D. I., Marsh, G. P., Felix, R., Brasher, B., Cooper, J., ... & Spencer, J. (2025). Structure-guided design of ISOX-DUAL-based degraders targeting BRD4 and CBP/EP300: a case of degrader collapse. Journal of Medicinal Chemistry, 68(9), 9638-9660. Degraders with dual activity against BRD4 and CBP/EP300 were designed. A structure-guided design approach was taken to assess and test potential exit vectors on the dual BRD4 and CBP/EP300 inhibitor, ISOX-DUAL. Candidate degrader panels revealed that VHL-recruiting moieties could mediate dose-responsive ubiquitination of BRD4. A panel of CRBN-recruiting thalidomide-based degraders was unable to induce ubiquitination or degradation of target proteins. High-resolution protein cocrystal structures revealed an unexpected interaction between the thalidomide moiety and Trp81 on the first bromodomain of BRD4. The inability to form a ternary complex provides a potential rationale for the lack of degrader activity with these compounds, some of which have remarkable affinities close to those of (+)-JQ1, as low as 65 nM in a biochemical assay, vs 1.5 μM for their POI ligand, ISOX-DUAL. Such a “degrader collapse” may represent an under-reported mechanism by which some putative degrader molecules are inactive with respect to target protein degradation. Accordion Item 2 Description text goes here Accordion Item 3 Description text goes here
